MiR-28-3p regulates high glucose-induced endothelial dysfunction by targeting CXXC5

Introduction Introduction: Data from the CEO database shows microRNA (miR) -28-3p levels are elevated in diabetic patients. Nevertheless, role of miR-28-3p peripheral artery disease with diabetes have not been investigated. Material and methods The level miR-28-3p, CXXC5, CXXC-type zinc finger protein 5, CXXC5’s downstream molecules as well endothelial function were Dual luciferase analyses used to confirm binding site CXXC5. Results Results: Under high-glucose condition, expression is increased whereas CXXC5 downregulated. Overexpression cell apoptosis inhibited proliferation, migration, vessel formation, whilst inhibiting its had opposite effect. overexpression inhibition could also influence both mRNA known molecules. Analysis bioinformatics data revealed a potential for demonstrated that suppressed by targeting 3'-untranslated region (3'-UTR) Following that, we overexpressed signaling decreased alone. As anticipated, co-overexpression partially reversed effect mimics. Conclusions Conclusion: These findings indicated regulated high glucose-induced dysfunction